EFFICACY OF BACTERIAL ISOLATES FROM SOIL FOR L-PHENYLALANINE (L-PHE) PRODUCTION IN DIFFERENT FERMENTATION MEDIA
M. Hassan, S. Nadeem*, K. Khaliq and I. Perveen
Deptt. of Zoology, Deptt. of Bio-Informatics*, Govt. College University, Faisalabad
Corresponding author e-mail: snadeem63@yahoo.com
ABSTRACT
Majority of the microbes have a natural ability to convert complex organic compounds into aromatic amino acids. Fifty-three isolates were obtained from soil on Nutrient agar medium through standard selection procedures. These isolates were grown on four different fermentation media containing either cane molasses, corn steep liquor (CSL), glucose or meat extract, and studied for phenylalanine production in the fermentation broths. Nineteen among them produced phenylalanine in CSL, whereas seven in meat extract medium (FM-1). Out of forty-nine percent of the producers, NIAB NFS-3 appeared to be the most prominent with 5.8 g phenylalanine/l.
Key words: Soil, phenylalanine production, fermentation media. ibt
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