PRODUCTION OF GOAT POX VIRUS VACCINE FROM A LIVE ATTENUATED GOAT POX VIRUS STRAIN
F. Abbas, F. A. Khan, A. Hussain*, F. Ahmed*, M. Ahmed, S. Ahmed*, M. Z. Ahmed, M. A. Attique, A. Wadood
and M. K. Taj.
Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistanistan
* Veterinary Research Institute, Quetta, Balochistan
Corresponding author’s email: email@example.com
Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days. Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers. Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation. TCID50 calculated for the pool virus was 10 5.5/ ml. Vaccine was found free from any bacterial and mycoplasma contamination. All mice and guinea pigs were found healthy after 21days post vaccinal administration. Vaccinated goats challenged after 14 days with 1 ml of virulent goat pox virus (TCID50 104.5/ml) did not show any unwanted reaction while controls challenged with 1 ml of virulent goat pox virus (TCID50 10 3.5/ml) showed pox lesions and rise in body temperature. The main objective of this study was to produce and study the efficacy of this vaccine.
Key words: attenuated, goat pox virus, confluent, monolayer, virulent, challenge.