RT Journal T1 MOLECULAR Characterization of Salmonella enterica SEROVARS in Broiler Chickens at Kafr El-Sheikh Governorate, Egypt A1 Amgad A. Moawad A1 Ahmed M. Ammar A1 Nagwa S. Rabie A1 Mohamed R. Sherief A1 Ibrahim E. Eldesoukey JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 32 IS 6 SP 1580 OP 1589 YR 2022 FD 2022/11/20 DO DOI https://doi.org/10.36899/JAPS.2022.6.0567 AB

Salmonella spp. are one of the most frequently reported foodborne pathogens worldwide. The present study investigated the virulence genotypes and antimicrobial sensitivity of Salmonella spp. isolated from broiler chickens in Kafr El-Sheikh governorate, Egypt. A total of 180 samples [liver (n=70), spleen (n=70) and caecum (n=40)] collected from 50 different broiler chicken flocks were used to isolate Salmonellae. All suspected samples were examined through a series of conventional bacteriological, biochemical, and serological techniques for isolation and identification of Salmonella spp. All isolates were tested for susceptibility to 16 antimicrobials. Virulence factors were determined using the polymerase chain reaction assays targeting the invA, pefA, avrA, sopB and spvC. The overall isolation percentage of Salmonella was 6.1%. Eleven Salmonella isolates belonging to four different serovars were recovered. S. Belgdam was the most predominant species (7/11, 63.6%), followed by S. Typhimurium (2/11, 18.2%), and S. Virchow and Salmonella enterica subsp. Salamae (S. Salamae) (1/11, 9.1% each). All the isolates were positive for all tested genes except S. Salamae, which harbored neither the sopB nor avrA genes. All isolates exhibited resistance to almost all antimicrobials used. The finding of the present study show high positivity of virulence genes as well as multidrug resistance of all serotypes suggesting pathogenic Salmonella strains.

K1 Salmonella; Chicken; cPCR; Virulence genes; Antimicrobials PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=VS-21-0075