RT Journal
T1 ANTIMICROBIAL POTENTIAL OF TEA TREE, CLOVE, BASIL AND THYME ESSENTIAL OILS AGAINST BACTERIAL ISOLATES OF BOVINE WOUNDS
A1 F. Leghari
A1 A. A. Kamboh1
A1 R. A. Leghari
A1 N. A. Korejo
A1 K. K. Malhi
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 31
IS 5
SP 1270
OP 1276
YR 2021
FD 2021/08/07
DO DOI https://doi.org/10.36899/JAPS.2021.5.0327
AB <p>The present research aimed to investigate the individual and combined antimicrobial potential of plant essential oils (EOs) viz., tea tree (<em>Melaleuca alternifolia</em>), clove (<em>Syzygium aromaticum</em>), basil (<em>Ocimum basilicum</em>), and thyme (<em>Thymus vulgaris</em>) against bovine wound isolates.&nbsp;<em>Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Escherichia coli&nbsp;</em>and&nbsp;<em>Klebsiella spp.</em>&nbsp;were isolated from bovine wound samples. Inhibition of microbial growth via disk diffusion method and minimum inhibitory concentration (MIC) was analyzed for EOs and standard antibiotics both individually and in combination. Tea tree and clove produced significantly higher (p&le;0.05) inhibitory effects against all the bacteria compared to one or other EOs and standard antibiotics. Basil+ neomycin, thyme+ ampicillin and tea tree+ ampicillin exhibited synergisms against all the wound isolates except&nbsp;<em>Klebsiella spp.&nbsp;</em>The MIC result indicated that clove followed by basil and tea tree exhibited significantly lower (p&le;0.05) MIC values against tested isolates compared to one or other EOs and antibiotics. The combination of thyme neomycin and clove norfloxacin exhibited a significantly lower (p&le;0.05) MIC against&nbsp;<em>S.pyogenes&nbsp;</em>and&nbsp;<em>E.coli&nbsp;</em>&nbsp;compared to other combination of EOs and antibiotics. Likewise, the combination of basil oxytetracycline and clove norfloxacin exhibited a significantly lower (p&le;0.05) MIC against&nbsp;<em>S.pneumoniae</em> compared to one or other combinations of EOs and antibiotics. These results demonstrated the significant antimicrobial potential of EOs against wound isolates; individually tea tree and clove showed superior effects, whereas combinations of basil+ neomycin, thyme+ ampicillin and tea tree+ ampicillin showed synergisms compared to one or other EOs and antibiotics.</p>
K1 antibacterial, bovine wound, plant essential oil, synergistic effect
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=VS-19-0092