RT Journal
T1 COMPARATIVE EVALUATION OF REAL-TIME PCR WITH CONVENTIONAL PCR ASSAY FOR DIAGNOSIS OF BRUCELLOSIS IN RUMINANTS
A1 M. Z. Saleem1
A1 R. Akhtar
A1 A. Aslam
A1 M. I. Rashid
A1 Z. I. Chaudhry
A1 B. A. Shah
A1 R. Ahmed
A1 M. Akmal
A1 G. Murtaza
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 30
IS 6
SP 1389
OP 1394
YR 2020
FD 2020/08/03
DO DOI https://doi.org/10.36899/JAPS.2020.6.0159
AB <p>Brucellosis is zoonotic and highly infectious disease which not only causes the economic losses in ruminants but also infect the humans. In the present study, we compared the molecular techniques of PCR versions and clinical specimens for the diagnosis of brucellosis in ruminants. Blood and serum samples were collected from 692 cattle, 798 buffalo, 471 sheep and 960 goats from two areas; Kasur and Sheikhupura. After serological screening with Rose Bengal antigen, the seropositive serum samples of 73 cattle, 61 buffalo, 91 sheep and 118 goats were subjected to Real-time PCR and Conventional PCR for comparison. Real-time PCR detected significantly (<em>P&le; 0.01</em>) more&nbsp;<em>Brucella abortus</em>&nbsp;(66 cattle, 53 buffalo) and&nbsp;<em>Brucella melitensis&nbsp;</em>(59 sheep, 81 goats) in large and small ruminants, respectively in the serum samples of seropositive ruminants than conventional PCR (45 cattle, 37 buffalo, 34 sheep and 47 goats). The&nbsp;<em>Chi</em>-square analysis confirmed significantly (<em>P&le; 0.01</em>) more detection of&nbsp;<em>B. abortus</em>&nbsp;(66 cattle, 53 buffalo) and&nbsp;<em>B. melitensis</em> (59 sheep, 81 goats) in serum samples specimens of cattle, buffalo and sheep, goats, respectively than their respective blood samples (48 cattle, 39 buffalo, 44 sheep and 63 goats) in seropositive ruminants. It can be concluded that real-time PCR assay is more sensitive and reliable method for the diagnosis of brucellosis, that serum is the optimal specimen for the diagnosis of brucellosis by Real-time PCR.</p>
K1 Brucellosis, B. abortus, B. melitensis, Buffalo, Cattle, PCR, RBPT, Molecular diagnosis.
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=VS-18-0044