RT Journal T1 PHYTOCHEMICAL ANALYSIS AND IN VITRO INVESTIGATION OF ANTI-INFLAMMATORY AND XANTHINE OXIDASE INHIBITION POTENTIAL OF ROOT EXTRACTS OF BRYOPHYLLUM PINNATUM A1 A. Latif1 A1 K. Ashiq A1 S. Ashiq A1 E. Ali A1 I. Anwer1and S. Qamar JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 30 IS 1 SP 219 OP 228 YR 2020 FD 2020/01/02 DO DOI https://doi.org/10.36899/JAPS.2020.1.0025 AB

The aim of present study is to emphasize the phytochemical and biological studies of root extracts of Bryophyllum pinnatum, as little work was documented on the roots. Phytochemical analysis of powder and extracts were evaluated by the standard methods. UV/ Visible scanning, FTIR and atomic absorption spectroscopy were also performed for the purposes of standardization. In vitro anti-inflammatory investigation was done by protein denaturation method and xanthine oxidase inhibition assay was performed to confirm anti-gout effect. Phytochemical analysis results revealed the presence of primary metabolites (%w/w); carbohydrates (48.54+0.6) > proteins (21.06+0.6) > lipids (1.32+0.001). Secondary metabolites (mg/g) were also isolated; methanol extract has higher concentrations of flavonoids (95.81+0.026) and glycosaponins (39.9+0.103) while in water extract polysaccharide (139.4 + 0.026) and polyphenols (114.93+0.0234) were found to be in maximum quantities as compared to the other extracts. UV/Visible and FTIR scanning results have shown the presence of various significant functional groups. In vitro anti-inflammatory evaluation showed that aqueous extract (87.46%) was found to be the most active with IC50 (570.24ug/ml) as compared to the reference drug diclofenac sodium. In vitro xanthine oxidase inhibition activity was performed and methanol extract (88.24%) found to be the most effective. The findings confirm the plant nutritional value and rationale behind its use in traditional medicine for the treatment of inflammation and gout. 

K1 Bryophyllum pinnatum, anti-inflammatory, gout, xanthine oxidase, phytochemical analysis PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=Pharm-18-0018