RT Journal
T1 VIABILITY OF MICROENCAPSULATED PROBIOTICS (LACTOBACILLUS REUTERI) IN GUAVA JUICE
A1 M. S. Javed
A1 M. Amir
A1 A. Amjad
A1 M. Shah
A1 M. J. Anwar
A1 F. Ahmad
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 33
IS 3
SP 644
OP 654
YR 2023
FD 2023/06/18
DO DOI https://doi.org/10.36899/JAPS.2023.3.0657
AB <p>Probiotics play a pivotal role to reduce gastrointestinal problems by exerting a drastic effect on various pathogenic microflora of the colon.&nbsp;<em>Lactobacillus reuteri</em>&nbsp;CECT-925 loaded beads were prepared by emulsion containing sodium alginate and sesame seed oil. Encapsulation was done by spraying emulsion into a 0.5% solution of calcium chloride. Microencapsulated probiotics incorporated guava juice was assessed for physicochemical analysis at the 15-day interval for 60 days. The juice was tested for probiotics viability, titratable acidity, pH, total soluble solids and organoleptic properties. In the control sample, viable counts of encapsulated probiotics were reduced from 7.68 to 1.96 log10 CFU/ml while in T<sub>1</sub>, T<sub>2</sub>&nbsp;and T<sub>3</sub>&nbsp;the initial numbers 7.39, 7.7 and 7.87 were reduced to 5.97, 6.87 and 6.02 log10 CFU/ml respectively at the termination of the storage period. However, pH and sensory scores decreased while total soluble solids and titratable acidity increased. Results indicated that microencapsulation by sodium alginate in combination with sesame oil retained the viability of&nbsp;<em>Lactobacillus reuteri &gt;&nbsp;</em>90% in guava juice. The acceptability of the product was 82.04% till the end of the storage period.</p>
K1 Probiotics, Lactobacillus reuteri, Sodium alginate, Sesame seed oil, Microencapsulation, Guava juice, Viability, Gastrointestinal health, Calcium chloride, Emulsion, Storage stability, Physicochemical analysis, Titratable acidity, pH, Organoleptic properties
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=Food 21-0013