RT Journal T1 ACIDIFICATION OF PHYTASE TREATED DIET IMPROVES THE BODY COMPOSITION AND BONE MINERALIZATION OF JUVENILE ROHU (Labeo rohita) A1 S. Z. H. Shah1 A1 M. Fatima A1 M. Afzal A1 S. M. Hussain A1 H. Azmat A1 M. Bilal A1 T. Ahmed JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 31 IS 5 SP 1485 OP 1491 YR 2021 FD 2021/08/07 DO DOI https://doi.org/10.36899/JAPS.2021.5.0350 AB

A 3×3 factorial feeding experimental was performed to find out the role of citric acid and phytase supplementations and interaction in improving body chemical composition and bone mineralization by adding them in plant based diet of rohu (Labeo rohita) juveniles. At first, the basal diet having citric acid at three different levels was supplemented i.e. 0%, 1.5% and 3%, and then three different phytase levels (0 FTU kgˉ¹, 750 FTU kgˉ¹ and 1000 FTU kgˉ¹) were supplemented further with each level of citric acid. Hence, total 9 experimental diets were formulated in this feeding trial. The results revealed that the concentration of total ash, crude protein and dry matter content significantly increased (p≤0.05), when treated with citric acid and phytase. However, contents of crude fat showed a reduced level in the carcass of juveniles by feeding both additives. The supplementation of citric acid and phytase both significantly (p≤0.05) affected the bone mineralization of fish. An increasing trend was observed for the body proximate analysis and bone mineral concentrations with an increase of supplements level in the diet. Conclusively, the results of this experiment showed that the supplementation of citric acid and phytase in the diet of L. rohita improved body composition and bone mineralization.

K1 Phytase, citric acid, rohu (Labeo rohita), bone mineralization, body mineralization, body proximate composition PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=Fish- 19-0016