RT Journal
T1 AGRO-MORPHOLOGICAL VARIATION AND GENETIC DIVERSITY ASSESSMENT OF TUNISIAN SUNFLOWER (HELIANTHUS ANNUUS L.) ACCESSIONS USING MICROSATELLITE MARKERS
A1 T. Hosni
A1 H. Chennaoui Kourda
A1 S. Medimagh
A1 N. Omri Ben Youssef
A1 Z. Abbes
A1 H. Ben Salah
A1 M
A1 Kharrat
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 33
IS 2
SP 330
OP 344
YR 2023
FD 2023/03/24
DO DOI https://doi.org/10.36899/JAPS.2023.2.0624
AB <p>Sunflower (<em>Helianthus annuus</em>&nbsp;L.) is considered as one of the most important oilseed crops in the world.&nbsp;<span lang="AR-SA">&lrm;</span><span lang="EN-GB">In Tunisia, sunflower is cultivated in the Northern region of the country using local populations which are genetically not well studied.&nbsp;</span>This study was conducted to evaluate the genetic variation of 33 accessions (26 local and 7 introduced) using 23 agro-morphological traits and 15 simple sequence repeats (SSR) markers.&nbsp;<span lang="EN-GB">The variance analysis of the agro-morphological traits showed a significant variation among sunflower accessions. The</span>&nbsp;relationship among the sunflower accessions was also performed by using the&nbsp;<span lang="EN-GB">principal component analysis; the two first axes&nbsp;</span>explained 54.4% of the total variability and showed that the accessions spread into five groups.&nbsp;<span lang="EN-GB">The groups (G3 and G4) could be used to improve sunflower varieties with high performance especially for the diameter of the head and the weight of 1000 seeds characters.&nbsp;</span>Ten among the 15 SSR primers used revealed clear polymorphic bands and were able to amplify 29 alleles with an average of 2.9 alleles per locus. The percentage of total polymorphism, the polymorphism information content (PIC) and&nbsp;<span lang="EN-GB">the&nbsp;</span><span lang="EN-GB">dissimilarity coefficient&nbsp;</span><span lang="EN-GB">values varied from 50 to 100% with an average of 91%, from 0.35 to 0.75 with an average of 0.50, and</span><span lang="EN-GB">&nbsp;from 0.00&nbsp;</span>to 0.81 with an average of 0.41, respectively. Cluster analysis of the<span lang="EN-GB">&nbsp;SSR markers grouped accessions into 3 distinct groups.</span>&nbsp;A significant correlation was observed among SSR markers and morphological traits<span lang="EN-GB">.&nbsp;</span>These results found in this study may be helpful for improvement of sunflower breeding programs.</p>
K1 Helianthus annuus; genetic diversity; microsatellite; morphological characterisation; cluster analysis
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=Biot-21-0036