RT Journal
T1 GENETIC DIVERSITY AND POPULATION STRUCTURE OF WILD RADISH IN EAST ASIA
A1 Q. Hana
A1 J. Lib
A1 H. Shena
A1 S. Sakaguchic
A1 Y. Isagid
A1 H. Setoguchic
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 32
IS 4
SP 1110
OP 1119
YR 2022
FD 2022/07/30
DO DOI http://doi.org/10.36899/JAPS.2022.4.0515
AB <p>Wild radish (<em>Raphanus sativus</em>&nbsp;L. var.<em>&nbsp;hortensis</em>&nbsp;f.&nbsp;<em>raphanistroides</em>&nbsp;Makino) is widely distributed in East Asia; however, its genetic diversity and population structure remain poorly understood. In our study, DNA sequence and microsatellite loci were used to analyze wild radish populations across the whole East Asia. Population genetic diversity analysis showed thatthe East Asian wild radish populations generally had high genetic diversity (average&nbsp;<em>H</em><sub>O</sub>=0.448), distinct population structure, and a positive correlation between genetic distance and geographic distance. AMOVA analysis suggested the genetic diversity among individuals in populations by far exceeded the diversity among populations/groups. The outcrossing breeding system, long geographic distance between populations and gene flow from cultivated radish populations may explain the high level of genetic diversity within wild populations. Bayesian cluster analysis identified four genetic clusters in the East Asian wild radish populations, observed by DNA sequencing analysis. The possible factors would be various aspects, such as oceanic barrier, climate fluctuations, and pollinators&rsquo; characteristics. This study provides a reference for the utilization of wild radish germplasm resources and the improvement of radish varieties in East Asia.</p>
K1 Wild radish, Genetic diversity, Population structure, DNA sequencing, Microsatellite loci
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=Biot-21-0014