RT Journal T1 MOLECULAR CHARACTERIZATION AND BIOLOGICAL CONTROL POTENTIAL OF AN EGYPTIAN ISOLATE OF SCLEROTINIA SCLEROTIORUM MITOVIRUS 1 A1 Mahmoud E. Khalifa A1 Eldessoky S. Dessoky A1 Atef S. Sadik JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 32 IS 4 SP 1101 OP 1109 YR 2022 FD 2022/07/30 DO DOI NA AB

Several mycoviruses have potential to induce hypovirulence on their fungal pathogens and therefore the interest in mycoviruses has increased in recent years. In the current study, a single double-stranded RNA (dsRNA) molecule of 2531 nts was detected, sequenced and characterized from an Egyptian isolate (D7) of Sclerotinia sclerotiorum fungus. The dsRNA has one open reading frame (ORF), in its positive strand, encoding a protein with conserved motifs characteristic of viral RNA-dependent RNA-polymerases (RdRps). The RdRp encoded by the ORF shares 91.84% identity with that of isolate HC025 of sclerotinia sclerotiorum mitovirus 1 (SsMV1) and consequently it was tentatively named SsMV1-D7. As for previously described mitoviruses, the termini of the (+) strand of SsMV1-D7 RNA could potentially fold into stable secondary structures. Horizontal transmission and virulence experiments showed that SsMV1-D7 is probably responsible for the altered growth and virulence of S. sclerotiorum.

K1 dsRNA; Mycovirus; Mitoviridae; Mitovirus; Sclerotinia sclerotiorum; Hypovirulence PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=Biot-20-0065