RT Journal T1 COMPARISON OF PHYTOCHEMICALS AND ANTIOXIDANT CAPACITY OF HYPERICUM PERFORATUM; WILD PLANT PARTS AND IN VITRO SAMPLES A1 C. Yaman 1 A1 Ş. Önlü A1 H. A. A. Ahmed A1 R. Erenler JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 32 IS 2 SP 596 OP 603 YR 2022 FD 2022/03/15 DO DOI http://doi.org/10.36899/JAPS.2022.2.0459 AB

The aim of study is to compare the phytochemicals and free radical scavenging activities in methanol extracts of flower, leaf, stem in vitro plantlet, callus of Hypericum perforatum L. In vitro cultures of H. perforatum was established by using MS-B5 medium contained plant growth regulators such as BAP, TDZ and picloram. Total phenolics and flavonoids were analysed by spectrophotometric methods.The stem was the richest in total phenolics (228.9 mg GAE/g extract) and flavonoids (102.4 mg QE/g extract). Quinic acid, gallic acid, (+)-catechin, ferulic acid, vanillic acid, p-coumaric acid, caffeic acid, and quercetin were determinated by LC-MS/MS. Free radical scavenging activities (ABTS and DPPH) of all samples were detected as IC50 values, and was compared to standards such as trolox and ascorbic acid. As a result, the stem exhibited the stronger antioxidant activities than other samples, and vanillic acid, ferulic acid and gallic acid could be produced by in vitro culture.

K1 Hypericum perforatum, antioxidant activity, in vitro plantlets, phytochemicals PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=Biot-20-0026