RT Journal
T1 ASSOCIATIONS BETWEEN PRL/RsaI POLYMORPHISM AND SOME PERFORMANCE TRAITS IN HOLSTEIN CATTLE REARED UNDER ORGANIC CONDITION
A1 M. Ozdemir
A1 Z. Sonmez
A1 V. Aksakal
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 31
IS 3
SP 900
OP 905
YR 2020
FD 2020/11/09
DO DOI https://doi.org/10.36899/JAPS.2021.3.0279
AB <p>The aim of this study was to ascertain the&nbsp;<em>RsaI</em>&nbsp;polymorphism of the&nbsp;<em>Prolactin</em>&nbsp;(<em>PRL</em>) gene of 186 organically reared Holstein cattle by PCR-RFLP method, in order to reveal the genetic variation of the population, and to reveal the relationship between&nbsp;<em>PRL</em>/<em>RsaI</em>&nbsp;genotypes and some milk yield traits. In the study, the&nbsp;<em>RsaI<sup>-/-</sup></em>&nbsp;genotype frequency,&nbsp;<em>RsaI<sup>+/+</sup></em>&nbsp;genotype frequency and&nbsp;<em>RsaI<sup>-/+</sup></em>&nbsp;genotype frequency of Holstein cattle were determined as 0.26, 0.22 and 0.52, respectively. The&nbsp;<em>RsaI<sup>-&nbsp;</sup></em>gene frequency was 0.52, and the&nbsp;<em>RsaI</em><sup>+</sup>&nbsp;gene frequency was 0.48. Hardy-Weinberg genetic equilibrium test showed that the distribution of genotype frequencies was determined to be in genetic equilibrium. The relationships between&nbsp;<em>PRL</em>/<em>RsaI</em>&nbsp;polymorphism and the examined milk yield traits were not significant. As a result, an economic and safe method PCR-RFLP for the detection of genetic markers, was successfully used in the determination of the 3 genotypes (<em>RsaI<sup>-/-</sup></em>,&nbsp;<em>RsaI<sup>-/+</sup></em>, and&nbsp;<em>RsaI<sup>+/+</sup></em>) of Prolactin gene in Holstein cattle reared under organic condition, and the results was similar with previously reported literature.</p>
K1 Prolactin, PCR-RFLP, polymorphism, marker, cattle
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=AS-18-0138