RT Journal
T1 ZMFLS1 PROMOTES OXIDATIVE STRESS RESISTANCE BY REGULATING FLAVONOL BIOSYNTHESIS IN TRANSGENIC ARABIDOPSIS THALIANA
A1 Y. Fan1
A1 H. Li2
A1 R. Fan
A1 H. Zhang
A1 X. Zhao
A1 B. Li1
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 31
IS 6
SP 1652
OP 1658
YR 2021
FD 2021/11/20
DO DOI https://doi.org/10.36899/JAPS.2021.6.0369
AB <p>Many biological and abiotic stresses can lead to excessive reactive oxygen species accumulation and oxidative damage in plants. The purpose of the present study was to elucidate the function of maize flavonol synthase 1 (ZmFLS1) in alleviating oxidative stress. The coding sequence of&nbsp;<em>ZmFLS1</em>&nbsp;was cloned from maize B73 complementary DNA and was introduced into&nbsp;<em>Arabidopsis thaliana</em>. Two&nbsp;<em>ZmFLS1</em>&nbsp;overexpression lines, OE-6 and OE-8, showed a significant increase in the accumulation of flavonoids, especially flavonols. The antioxidant capacity test showed that overexpression of&nbsp;<em>ZmFLS1</em>&nbsp;resulted in a higher oxygen radical scavenging rate. Consistent with those, transgenic plants with&nbsp;<em>ZmFLS1</em>&nbsp;overexpression showed enhanced oxidative stress tolerance and lower oxidative damage. In conclusion, ZmFLS1 promoted plant oxidative stress tolerance through regulating flavonoid biosynthesis and accumulation, especially flavonols, and&nbsp;<em>ZmFLS1</em> has the potential in mitigating oxidative damage caused by environmental stress in plants.</p>
K1 ZmFLS1; Flavonols; Oxidative stress; MDA (Malondialdehyde)
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=AG-20-0052