RT Journal T1 TISSUE CULTURE OPTIMIZATION OF PODOPHYLLUM HEXANDRUM L., AN ENDANGERED MEDICINAL PLANT A1 Z. Zuhra A1 D. Saleem A1 W. Akhtar A1 T. Mahmood1 JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 31 IS 2 SP 488 OP 499 YR 2020 FD 2020/10/03 DO DOI https://doi.org/10.36899/JAPS.2021.2.0238 AB

hexandrum (Bankhakri) is an important medicinal plant due to its useful active compounds such as podophyllotoxin, a precursor of anti-cancer drugs. Currently, it is carrying the status of endangered species in Pakistan, India, China and Afghanistan due to slow growth and overharvesting. The present study is an attempt to establish an efficient protocol for callogenesis and organogenesis of P. hexandrum. Fresh fruits and rhizomes were collected from natural habitats. Seeds were sterilized and germinated for callogenesis and organogenesis. The present study describes that leaf explants resulted in the highest callus response (100%) followed by stem (90%) on Murashige and Skoog media (MS) media supplemented with 6.75 µM Naphthalene acetic acid (NAA) and 11.37 µM 6-Benzylaminopurine (BAP). Moreover, 1.0 µM indole acetic acid (IAA) in combination with 5.0 µM BAP successfully induced 80% callogenesis from root explants while 2,4 Dichloro-phenoxy acetic acid (2,4-D) at 3.0 and 4.0 µM resulted in 20% calli from embryos. Principal component analysis (PCA) showed that leaf and stem explants revealed considerable callus response in combination of NAA+BAP. Rhizome did not support callogenesis rather direct shooting was achieved with a combination of IAA (10.42-11.42 µM) and activated charcoal 5 g/L. ANOVA showed the most significant variability (P = 0.007198) from rhizome explant in direct regeneration.

K1 Podophyllum hexandrum L., Tissue culture, Conservation, Callogenesis, Organogenesis PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=AG-19-0327