RT Journal T1 CYTOKINE AND OXIDATIVE STRESS PROFILES IN CERVICOVAGINAL MUCUS OF COWS AT INSEMINATION: ASSOCIATIONS WITH PREGNANCY AND CALF SEX A1 Mehmet Akif Kilinç A1 Ali Risvanli JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 35 IS 5 SP 1370 OP 1377 YR 2025 FD 2025/09/30 DO DOI https://doi.org/10.36899/JAPS.2025.5.0117 AB

This study aimed to examine the relationship between the Th1/Th2 cytokine balance and oxidative stress parameters in the cervicovaginal mucus of cows at the time of insemination. It also investigated the potential associations of these factors with conception rates and calf sex. Cervicovaginal mucus samples were collected from 90 cows at the time of insemination. Of these, 55 cows were confirmed pregnant (Group I), and 33 were non-pregnant (Group II). Ultimately, 35 female and 20 male calves were born. The mucus samples were analyzed for interleukins (IL) -2, IL -4, IL -5, IL -10, tumor necrosis factor-alpha (TNFα), interferon-gamma (IFN-γ), and oxidative stress markers including malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD). IFN-γ levels were higher in pregnant cows, while MDA levels were higher in non-pregnant cows. Among parturient cows, those that gave birth to male calves had higher levels of IL-2 and GSH compared to those with female calves. Based on these findings, cytokine and oxidative stress parameters in cervicovaginal mucus may not serve as reliable biomarkers for predicting pregnancy success or calf sex. Furthermore, no significant differences in Th1/Th2 cytokine balance were observed in relation to pregnancy status or offspring gender.

K1 Cow, cervicovaginal mucus, cytokine, oxidative stress PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2025-JAPS-95