RT Journal
T1 EFFECTS OF SYNBIOTIC FROM NONI LEAF EXTRACT SUPPLEMENTATION ON SURVIVAL, GROWTH, AND RESISTANCE TO Vibrio harveyi IN THE WHITELEG SHRIMP, Litopenaeus vannamei
A1 Thi Cam Tu Phan
A1 Truong Giang Huynh
A1 Thi Kim Lien Nguyen
A1 Thi Thanh Hien Tran
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 36
IS 5
YR 2026
FD 2026
DO DOI https://doi.org/10.36899/JAPS.2026.5.0104
AB <p class="MsoNormal" style="text-align: justify; line-height: 1.1; margin: 12pt 0in 0in;"><span style="font-size: 12pt; font-family: 'Times New Roman', serif;">This study assessed the effects of noni leaf and <em style="mso-bidi-font-style: normal;">Lactobacillus plantarum</em> CMT1 on survival, growth, and resistance to <em style="mso-bidi-font-style: normal;">Vibrio harveyi </em>of whiteleg shrimp, <em>Litopenaeus vannamei,</em> by improving digestive function and immunity. The experiment was conducted in a recirculating aquaculture system using a completely randomized design with five treatments and three replicates per treatment. One thousand five hundred healthy shrimp were assigned to 5 diet groups for a 60-day feeding period, including a control (commercial feed), diet 1 (commercial feed + 0.5% noni leaf), diet 2 (commercial feed + 1% noni leaf), diet 3 (commercial feed + 0.5% noni leaf + 10<sup>8</sup> CFU kg<sup>-1</sup> of <em style="mso-bidi-font-style: normal;">L. plantarum</em> CMT1), and diet 4 (commercial feed + 1% noni leaf + 10<sup>8</sup> CFU kg<sup>-1</sup> of <em style="mso-bidi-font-style: normal;">L. plantarum</em> CMT1). One-way ANOVA analysis showed that, compared with the control diet, other diets greatly enhanced growth indices of shrimp, with the highest growth observed in diet 3 (p &le; 0.05). Supplementation with diets 1, 2, 3, and 4 improved the abundance of <em style="mso-bidi-font-style: normal;">Lactobacillus</em> spp. and decreased <em style="mso-bidi-font-style: normal;">Vibrio</em> spp. in the shrimp intestine. Shrimp fed diets 1 and 3 showed significantly higher amylase activity than those in the control and in diets 2 and 4. In addition, shrimp fed diet 3 group showed greatly enhanced survival, increasing from 78.7% to 88.3%, and improved immune parameters after <em style="mso-bidi-font-style: normal;">Vibrio harveyi</em> challenge compared to other experimental diets and the control. In summary, the administration of 0.5% noni leaf and 10<sup>8</sup> CFU kg<sup>-1</sup> of <em style="mso-bidi-font-style: normal;">L. plantarum</em> CMT1 increased growth performance, improved digestive function, and immunity system as well as promoted the survival rate against <em style="mso-bidi-font-style: normal;">V. harveyi </em>in shrimp. </span></p>
K1 Morinda citrifolia, leaf extract, Litopenaeus vannamei, synbiotic, digestive enzyme
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2025-JAPS-866