RT Journal
T1 Diverse expression of isoflavonoid-related genes based on transcriptomic datasets of Pueraria mirifica cultivars
A1 Huynh Thi Thu Hue
A1 Nguyen Thi Bich Ngoc
A1 Hanh Hong Ha
A1 Oanh Thi Kieu Pham
A1 Canh Xuan Nguyen
A1 Hoang Huy Nguyen
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 36
IS 4
YR 2026
FD 2026
DO DOI https://doi.org/10.36899/JAPS.2026.4.0100
AB <p class="Keyword" style="margin-top: 12pt; line-height: normal; text-align: justify;"><span lang="EN-GB" style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;">White Kwao Krua, also known as <em>Pueraria mirifica</em>, is a traditional medicinal plant in several Asian countries. This plant has a high content of essential phytoestrogens such as isoflavones and chromenes, particularly miroestrol. However, </span><span style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext;">their biosynthetic pathway remains unclear and is currently under investigation</span><span lang="EN-GB" style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;">. Three gene families Chalcone isomerases (<em>CHI</em>s), Chalcone synthases (<em>CHSs</em>), and </span><span lang="TR" style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: TR;">UDP-dependent glycosyltransferases</span><span lang="TR" style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;"> </span><span lang="EN-GB" style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;">(<em>UGTs</em>) play a key role in the phytoestrogen biosynthesis in <em>P. mirifica</em>. They are large gene families with myriad members, involved in several plant functions. In the research, five <em>P. mirifica</em> cultivars (</span><span style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext;">TLBYT, TLCNX, TLDB, NA, and SL)</span><span style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;"> <span lang="EN-GB">were cultivated under the same conditions and then their leaf, stem, and tuber tissues were collected. The transcriptomes of the cultivars were sequenced, assembled, and annotated in the research. </span></span><span lang="TR" style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: TR;">By using RNA-seq, the t</span><span style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext;">ranscriptome assembly yielded over 300,000 unigenes, of which approximately 32,000 were annotated across four major databases:<em> </em>NCBI-Nr (229569 unigenes), SwissProt (158667 unigenes), COG (112089 unigenes), and KEGG (61480 unigenes). </span><span lang="EN-GB" style="mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'Times New Roman'; color: windowtext; mso-ansi-language: EN-GB;">Seventeen individuals from these gene families that may catalyze or influence miroestrol and isoflavone biosynthesis were detected. The RT-qPCR analysis revealed tissue-specific gene expression, with several genes showing preferential expression in either leaves or tubers. <em>CHS11</em>, <em>CHS13</em>, and <em>UGT74</em> gene were predominantly expressed in leaves, whereas <em>CHI4A</em>, <em>CHI3A2</em>, and <em>CHS14</em> showed higher expression in tubers, the primary site of phytoestrogen accumulation. These results provide transcriptomic data for different <em>P. mirifica</em> varieties and demonstrate tissue-specific expression patterns of key <em>CHI</em>, <em>CHS</em>, and <em>UGT</em> genes involved in the isoflavonoid biosynthetic pathway.</span></p>
K1 Chalcone isomerase, Chalcone synthase, phytoestrogens, Pueraria mirifica, UDP-dependent glycosyltransferases.
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2025-JAPS-770