RT Journal T1 ANTHOCYANIN ACCUMULATION AND GENE EXPRESSION ANALYSIS IN BLACK CARROT CALLI UNDER SALT AND SALICYLIC ACID STRESSES A1 Berfin Odabasi A1 Sedanur Sebikbay A1 Asiyenur Üner A1 Gizem Yildirim Bastemur A1 Murat Pekmez A1 Sabriye Perçin Özkorucuklu A1 Ozgur Cakir JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 35 IS 2 SP 445 OP 457 YR 2025 FD 2025/04/28 DO DOI https://doi.org/10.36899/JAPS.2025.2.0037 AB

Black carrot (Daucus carota ssp. sativus var. atrorubens Alef.), is an important crop plant from the Apiaceae family, rich in anthocyanins, which contributes to its antioxidant capacity. Anthocyanins are secondary metabolites give purple to red color to plants, aid to adapt plants to severe environmental conditions. The main purpose of this study is to investigate the effects of NaCl and salicylic acid (SA) stress conditions on anthocyanin accumulation and antioxidant activity of total anthocyanin extract from black carrot callus cultures. In this study, one month old calli elicited with 50, 100, 150, 200 mM NaCl and 50, 100, 200, 400 µM SA. qRT-PCR analyses were performed to examine gene expression in the phenylpropanoid pathway. The anthocyanin profiles of the extraction obtained from stressed purple carrot calli were determined through HPLC-DAD while antioxidant capacities were evaluated with DPPH and CUPRAC assays. Results indicated that SA application generally increased antioxidant capacity, with the highest activity observed particularly in calli treated with 150 mM NaCl on day 15. NaCl elicitation was more effective in inducing anthocyanin biosynthesis compared with SA. HPLC analysis demonstrated a notable increasement of anthocyanin accumulation in NaCl treated samples, dramatically on the 10th day with 200 mM NaCl. This study indicated that elicitation with NaCl and SA affected anthocyanin accumulation and antioxidant capacity in black carrot calli. Thus, it is suggested that optimized elicitation conditions can be used to provide an alternative approach to increase the amount of anthocyanins to utilize its valuable health-promoting properties. By exploring the molecular mechanisms of anthocyanin biosynthesis under NaCl and SA stress, future studies could shed light on understanding of plant defense, potentially contributing to the development of more resilient varieties.

K1 Anthocyanin, Antioxidant Activity, Daucus carota ssp., Elicitation, Gene Expression, HPLC PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2024-JAPS-2060