RT Journal
T1 COMPARATIVE ANALYSIS OF METABOLITES AND GENE EXPRESSION VARIABILITY IN SIX CONTRASTING POTATO VARIETIES: IMPLICATION FOR BREEDING AND NUTRITIONAL QUALITY
A1 Wen-Bin Luo
A1 Hua-Wei Li
A1 Guo-Chun Xu
A1 Aqib Sayyed
A1 Yong-Qing Xu
A1 Rong-Chang Ji
A1 Si-Xin Qiu
A1 Muhammad Qadir
A1 Hao Tang
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 35
IS 1
SP 68
OP 79
YR 2025
FD 2025/02/18
DO DOI https://doi.org/10.36899/JAPS.2025.1.0005
AB <p>As one of the four major staple crops, potato exhibits significant differences among varieties. Therefore, understanding the relationship between nutritional quality and molecular metabolism in potato varieties is essential for facilitating the development of the processing industry and improving dietary nutrition. In this study, six potato varieties: Mincaishu 1 (C1), Mincaishu 2 (C2), Mincaishu 3 (C3), Mincaishu 4 (C4), Minshu 2 (M2), and Minshu 3 (M3) were tested for metabolomics and transcriptomics analyses. The aim of this study was to investigate the molecular basis underlying the formation of distinctive traits in these varieties. The two varieties with the greatest differences in metabolites were C4 and C1, exhibiting disparities in 657 substances. These differential substances were primarily involved in metabolic pathways such as the synthesis and degradation of ketone bodies, the citrate cycle (TCA cycle), and alanine, aspartate, and glutamate metabolism. Further transcriptomic analysis revealed that the overall gene expression level in stems was lower than in leaves for all six varieties. However, the expression patterns of the same genes were consistent between leaves and stems across different varieties. The highest number of differentially expressed genes was observed in the leaves of C4 and C1. Additionally, through a correlation analysis of transcriptomic and metabolomic data, this study identified several shared pathways between the two omics approaches, including the citrate cycle and sulfur relay system metabolism. This study will serve as a cornerstone for advancing potato breeding and agricultural practices by improving yield, quality, and the metabolic profile of important potato traits, with our multi-omics analysis providing crucial insights into potato metabolism and facilitating the exploration of innovative breeding strategies.</p>
K1 Potato, Metabolomics, Transcriptomics, Citrate cycle, Potato breeding
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2024-JAPS-2018