RT Journal
T1 SNAKE VENOM ENZYMES NEUTRALIZATION POTENTIAL OF PHYTOCHEMICALS EXTRACTED FROM DATURA ALBA SEEDS
A1 Syeda Maryam Fatima
A1 Wajiha Tahir
A1 Mahera Moin
A1 Humera Waheed
A1 Hanzala Khan
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 34
IS 5
SP 1112
OP 1118
YR 2024
FD 2024/10/22
DO DOI https://doi.org/10.36899/JAPS.2024.5.0793
AB <p><em>Datura</em>&nbsp;is a flowering perennial herb, belonging to the family Solanaceae, is widely distributed in many countries including Pakistan, and other tropical and sub-tropical parts of the world. The present study explored the bioactivities of phytochemicals obtained from&nbsp;<em>Datura alba</em>&nbsp;n-hexane and n-butanol seed extracts. The seed extracts showed the presence of flavonoids, terpenoids and alkaloids while saponins were absent. The n-hexane extract showed 100% human salivary amylase and 19% snake venom PLA<sub>2</sub>&nbsp;(svPLA<sub>2</sub>) inhibition activity at examined lowest and highest concentrations respectively. It also exhibited mild (24% RSA) antioxidant activity at highest concentration (600 &micro;g/&micro;l) analyzed. On the other hand, salivary amylase inhibition and potent (80% RSA) antioxidant activity at 100 &micro;g/&micro;l concentration was observed in n-butanol extract. Interestingly, n-hexane extract showed snake venom protease while n-butanol extract showed svPLA<sub>2</sub>&nbsp;enhancing activity and no venom anti-protease activity. The study demonstrated that both n-hexane and n-butanol extracts are natural sources of antioxidant and antidiabetic compounds. The n-hexane extract showed venom PLA<sub>2</sub> neutralization which is reported for the first time.</p>
K1 Traditional medicine;Datura alba; Anti-phospholipase A2; Anti-amylase; Antioxidant
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2023-JAPS-905