RT Journal T1 THE DETECTION AND IDENTIFICATION OF THE CR1-LIKE MEMBRANE BINDING PROTEIN OF PORCINE ERYTHROCYTES A1 Zheng Zhang A1 Kuo-Hai Fan A1 Na Sun A1 Pan-pan Sun A1 Yao-Gui Sun A1 Hong-quan Li A1 Wei Yin JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 34 IS 5 SP 1324 OP 1332 YR 2024 FD 2024/10/22 DO DOI https://doi.org/10.36899/JAPS.2024.5.0814 AB

Erythrocyte complement receptor I-like (ECR1-like) is a natural immunoreactive molecule on the surface of porcine erythrocyte membrane. The aim of this study was to establish an effective method for the detection and characterization of porcine erythrocyte CR1-like membrane-bound proteins, and to explore their expression characteristics and biological significance in porcine erythrocyte membranes.The CR1-like protein ligands were observed by laser confocal microscopy using fluorescence immunocytochemistry with two types of PDZ-binding domain monoclonal antibodies, FAP-1 (Fas-associated phosphatase-1) and ZO2 (Tight Junction Protein ZO-2); meanwhile, immunoprecipitation and Western blot techniques were used to detect the membrane proteins of porcine blood cells. The immunofluorescence cytochemical staining showed that the specific fluorescence sites of CR1-like and FAP-1 molecules in the porcine erythrocyte membrane skeleton were identical; the sum of the difference squares of the site distances of 253 typical positive erythrocytes was 0.2224, indicating that the difference between the site distances of CR1-like and FAP-1 in each group was approximately 0. The results showed that the distribution of CR1-like and FAP-1 was consistent with a co-local relationship, and the FAP-1 molecule was clearly observed in the examined gel by immunoprecipitation. The results indicate that CR1-like does not bind directly to the erythrocyte membrane skeleton protein, but is distributed on the surface of the porcine erythrocyte membrane through the riveted structure of the FAP-1 protein molecule.

K1 CR1-like; FAP-1; Immunoadhesion; Porcine erythrocytes PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2023-JAPS-811