RT Journal T1 Alternaria arborescens, IDENTIFIED AS A LEAF NECROSIS PATHOGEN OF Vigna radiata IN PAKISTAN A1 Sobiya Shafique A1 Shazia Shafique A1 Ume Attia A1 Mariam Zameer JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 34 IS 3 SP 652 OP 661 YR 2024 FD 2024/05/31 DO DOI https://doi.org/10.36899/JAPS.2024.3.0751 AB

Vigna radiata (mung bean) is a most cultivated legume crop having high nutritive and clinical value. A survey was accompanied to isolate leaf spot pathogen from mung bean plants. Infected leaf samples were collected and a novel pathogen, Alternaria arborescens was isolated and identified on morphological and molecular basis. Molecular identification was done using nucleotide sequence analysis of rDNA internal spacer sequence (ITS), partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and elongation factor (EF) regions. Morphological characters demonstrated grayish-black, woolly, concentric rings on Malt Extract Agar (MEA) plates. The conidiophores were well defined; septate with terminal and sub-terminal branches having tan to brown, short ovoid or ellipsoid, and 7-11 µm sized conidia with transverse septation. In molecular characterization, BLAST analysis of the rDNA-ITS region of the pathogen, A. arborescens exhibited maximum (99%) homology with other A. arborescens GenBank strains. Similarly, 100% homology was found with partial glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and translation elongation factor. Afterward, Koch’s pathogenicity aptitude of the identified pathogen was confirmed by the occurrence of the same disease symptomology and re-isolation of identical organisms from artificially inoculated leaves in the in vitro and in vivo trials. The study signifies the novel documentation of A. arborescens as a leaf spot pathogen of mung bean in Pakistan. The manifestation of this pathogen could result in a serious economic impact on mung bean or might be a possible pathogen of other pulse crops if not managed in time.

K1 Alternaria arborescens, Genetic characterization, Leaf spot disease, Morphology, Vigna radiata PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2023-JAPS-1479