RT Journal
T1 ISOLATION AND STRUCTURAL ELUCIDATION OF PHENOLIC COMPONENTS FROM CALOTROPIS PROCERA (AIT) AND EVALUATION OF INSECTICIDAL ACTIVITY
A1 Bellaouar Nacira
A1 Bouziane Mebarka
A1 Laichi Yacine
A1 Hadjadj Mohamed
A1 Hammoudi Roukia
A1 Hadj Mahammed Mahfoud
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 33
IS 4
SP 971
OP 977
YR 2023
FD 2023/08/04
DO DOI https://doi.org/10.36899/JAPS.2023.4.0690
AB <p>The identification and characterization of two phenolic compounds from the ethyl acetate (EtAc) extract of&nbsp;<em>Calotropis procera&nbsp;</em>aerial parts were performed using chromatographic and spectroscopic methods namely Thin Layer Chromatography (TLC), Preparative Thin Layer Chromatography (PTLC), High-Performance Liquid Chromatography (HPLC), Infrared spectroscopy (FT-IR) and Nuclear Magnetic Resonance (<sup>1</sup>H-NMR,&nbsp;<sup>13</sup>C-NMR).Two major compounds were isolated to be the 3, 3&rsquo;, 4&rsquo;, 5, 7-pentahydroxy flavones-3-rutinoside (Rutin) and 3,4,5-trihydroxybenzoic acid(Gallic acid), the results were confirmed by comparison with standards. In addition, the insecticidal activity of these isolated compounds was determined against&nbsp;<em>Parlatoria blanchardi</em><em>targ&nbsp;</em>individuals, 71.73% and 59.44% mortality were recorded at 0.5 mg/ml of Rutin and Gallic acid respectively, after 24 h of treatment; whereas, the larval stages were the most sensitive to Rutin treatment (LD<sub>50&nbsp;</sub>equal to0.10 mg/ml for 1<sup>st&nbsp;</sup>larva and 0.18 mg/ml for 2<sup>nd</sup> larva).</p>
K1 Calotropis procera, Rutin, Gallic acid, Parlatoria blanchardi
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-612