RT Journal
T1 IN VITRO ANTICANCER (HELA), ANTI-INFLAMMATORY, BRINE SHRIMP LETHALITY ASSAY AND GC-MS ANALYSIS OF WHOLE PLANT ARCEUTHOBIUM OXYCEDRI (DWARF MISTLETOE) N-HEXANE FRACTION
A1 Ashif Sajjad
A1 Nazima Yousaf Khan
A1 Jahangir Khan Achakzai
A1 Muhammad Anwar Panezai
A1 Inam ul Haq
A1 Fouzia Noreen
A1 Ayeesha Masood
A1 Shazia Saeed
A1 Sajid Nabi
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 33
IS 3
SP 544
OP 552
YR 2023
FD 2023/06/18
DO DOI https://doi.org/10.36899/JAPS.2023.3.0647
AB <p>Mistletoe has been used for anticancer treatment in different parts of the world. Most of the chemotherapeutic drug molecules are isolated from mistletoe. The present study aims to comprehensively examined for the first-time whole extract and different fractions of&nbsp;<em>Arceuthobium oxycedri</em>&nbsp;(drawf mistletoe) belonged to Ziarat Juniper Forest of Balochistan, Pakistan. The whole plant extract and its different fractions were investigated for some biological activities such as: anticancer by MTT assay on HeLa cell line, Brine Shrimp Lethality by &beta;-hatching technique and Anti-inflammatory by chemiluminescence technique in oxidative burst assay. The structural elucidation was done by GC-MS and a total of 21 compounds were identified. The whole plant&nbsp;<em>A. oxycedriI,</em>&nbsp;n-hexane fraction (WAOHF), exhibited 69.3% mortality against cervical cancer cell line (HeLa) with IC<sub>50</sub>&nbsp;value of 11.4 &plusmn; 0.7. The brine shrimp lethality activity in WAOHF showed 53.3% mortality at highest concentration. The anti-inflammatory activity in whole plant&nbsp;<em>A. oxycedri</em>&nbsp;methanolic extract (WAOME), WAOHF and whole plant&nbsp;<em>A. oxycedri</em> aqueous fraction (WAOAF) were inactive. The obtained results revealed that the WAOHF exhibited significant anticancer against cervical cancer cell line (HeLa), which can be considered as an alternative therapeutic anticancer drug in future with least side effects.</p>
K1 Arceuthobium oxycedri; Anticancer; Anti-inflammatory; Brine Shrimp Lethality; n-hexane fraction
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-608