RT Journal
T1 POOLED MAPPING OF QUANTITATIVETRAIT LOCI ASSOCIATED WITH DROUGHT TOLERANCE IN RICE (ORYZA SATIVA L.) AT SEEDLING STAGE
A1 Yingbao Wu
A1 Gaoyang Zhang
A1 Chao Zhang
A1 Weiqi Tang
A1 Ting Wang
A1 Huining Zhang
A1 Hongkai Wu
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 33
IS 6
SP 1467
OP 1473
YR 2023
FD 2023/12/15
DO DOI https://doi.org/10.36899/JAPS.2023.6.0686
AB <p><span lang="EN-US">Drought is among the foremost constraints influencing global rice productivity. The drought tolerance nature of rice is complicated, depending on multiple components and having low heritability. Thus, breeding drought-tolerant varieties is a fundamental way which can be used to increase rice yield in drought. To investigate the genetic basis of seedling tolerance to drought stress of rice (<em>Oryza sativa&nbsp;</em>L.), we performed QTL mapping on a big F<sub>2</sub>&nbsp;population of 2600 participants from a cross between the&nbsp;<em>japonica</em>&nbsp;rice variety Huaidao 5 (HD5) and the&nbsp;<em>indica&nbsp;</em>rice variety 1892S&nbsp;</span><span lang="EN-US">through performing bulked segregant analysis and whole-genome sequencing (BSA-seq).</span><span lang="EN-US">&nbsp;HD5 showed greater tolerance to prolonged drought stress compared with1892S</span><span lang="EN-US">at seedling stage. By analyzing a pair of opposite DNA pools made from 182 extremely-sensitive seedlings and 182 extremely-tolerant seedlings from the F<sub>2</sub>&nbsp;population using the block regression mapping (BRM) method, we mapped a QTL on chromosome 1, of which the additive effect was estimated to explain 2.20% of the phenotypic variance. We named the QTL</span><em><span lang="EN-US">qSLDT1.1</span></em><span lang="EN-US">(<em>q</em>&nbsp;represents quantitative trait loci,&nbsp;<em>SL&nbsp;</em>represents seedling leaf,&nbsp;<em>DT&nbsp;</em>represents drought tolerance,&nbsp;<em>1.1</em></span><span lang="EN-US">representsthe first one found on chromosome 1</span><span lang="EN-US">), which must be a novel QTL, because no QTLs for rice seedling tolerance to drought stress have been mapped on chromosome 1 before. The information derived from the current research facilitates marker-assisted breeding of drought-resistant lines and positional cloning of the gene conferring drought tolerance in rice.</span></p>
K1 Rice, Drought tolerance, QTL mapping, Bulked segregant analysis, Whole-genome sequencing
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2022-JAPS-490