RT Journal T1 ANTIOXIDANT ENZYMES PROFILE DURING CRYOPRESERVATION OF NILI RAVI A1 BUFFALO BULL SPERMATOZOA (BUBALUS BUBALIS) S. Iqbal A1 A. Riaz A1 S. Naz A1 M. I. Rashid Syed M. H. Andrabi A1 N. Ahmad JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 29 IS 6 SP 1800 OP 1802 YR 2019 FD 2019/12/01 DO DOI NA AB

Present study was designed to ascertain antioxidative enzyme shift during cryopreservation of buffalo bull spermatozoa. Enzymatic profile of three enzymes (catalase, superoxide dismutase and glutathione peroxidase/reductase) was established for fresh, post-dilution, pre-freezing and post-thaw stages. A total of twenty semen samples from four bulls were cryopreserved in French straws by using tris based extender. Catalase (U/mL), superoxide dismutase (U/mL) and total glutathione (µM) profile for fresh semen (25.8±1.6, 93.1±1.3, 77.4±5.2; respectively), lowered with semen extension (6.0±0.6, 60.7±2.4, 63.0±0.9 respectively; P<0.05).During equilibration time, the enzymatic profile for catalase remained stable (7.0±0.7; P>0.05) but superoxide dismutase and glutathione profile deteriorated further (47.7±1.4, 44.6±0.6 respectively; P<0.05). During cryopreservation, major shift was observed in catalase profile (2.3±0.3; P<0.05) while superoxide dismutase and glutathione readings were stable in post-thaw evaluations (P>0.05). It is concluded that cryopreservation process deteriorated catalase enzyme profile while superoxide dismutase and glutathione profiles are declined by metabolic activities of spermatozoa in time dependent manner.

K1 Buffalo semen, Cryopreservation, Antioxidant enzyme activity PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-633