RT Journal T1 EFFICIENT IN VITRO BULBLET PRODUCTION OF ENDANGERED HYACINTHELLA Micrantha (B OISS.) CHOUARD A1 B. Bulut A1 S. Uranbey A1 H. A. A. Ahmed A1 M. Çali_kan JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 29 IS 4 SP 1135 OP 1141 YR 2019 FD 2019/08/01 DO DOI NA AB

Hyacinthella micrantha (BOISS.) Chouard with their attractive flowers is one of the important endemic and endangered species of Turkey. We aimed to improve a standard in vitro multiplication protocol using basal scales for bulblet production and conservation of the species. Bulb scales of the species collected from natural habitat were cultured in the different basal Murashige and Skoog (MS), Orchimax and Lindeman Orchid Medium embryonic callus induction medium. Then, they were transferred to MS, Orchimax and Lindeman Orchid Medium basal bulblet induction medium supplemented with cytokinins (1.0, 2.0, 4.0 and 8.0 mg/l N6-benzylamino-purine (BAP), Zeatin and 2-isopentyladenine (2-iP) at 16 °C. After ten months of culture initiation, high frequency bulblet regeneration was developed using bulb scales on different basal media containing different concentrations of cytokinintypes. The highest ratio of bulb scales forming shoot/bulblets per explant (87%) and bulblets per explants (5.60) were induced on a Orchimax media supplemented with 8 mg/L Zeatin. MS and Orchimax medium gave the best result in terms of embryonic calli formation and in vitro bulblet production compared to Lindeman Orchid medium. 2,4-D, BAP and Zeatin were more effective than2-iPfor in vitro bulblet production. Also, regenerated bulblets subjected to pre-cold treatments for 3 months at 4-6 ºC resulted in increasing of new mini bulblets (approximately 15%). Regenerated bulblets were acclimatized with high survival ratio (82 %).

K1 bulblets, Hyacinthella micrantha, in vitro, micropropagation PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-425