RT Journal
T1 MOLECULAR EXPRESSION OF CYCLIN DEPENDENT KINASE INHIBITOR (p21) I N CANINE TUMORS
A1 S. Manzoor
A1 R. Saif
A1 H. Sadia
A1 S. Firyal
A1 M. Tayyab
A1 M. Mansha
A1 A. K. Mahmood
A1 A. S. Hashmi A. R. Awan
A1 M. Wasim
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 29
IS 4
SP 1127
OP 1134
YR 2019
FD 2019/08/01
DO DOI NA
AB <p><em>p21</em>&nbsp;is a cyclin dependent kinase inhibitor, that plays an important role in cell growth, differentiation and apoptosis. The aim of present study was to analyze the mutation and expression of tumor suppressor gene (<em>p</em>21) in canine tumors. A total of 26 tumors (mammary tumors and mast cell tumors) and normal samples from different breed of dogs were analyzed by using direct DNA sequencing and quantitative RT-PCR for mutation and expression profiling of&nbsp;<em>p</em>21gene respectively. The coding exon of&nbsp;<em>p</em>21 gene (Exon2 and 3) did not show any polymorphism in studied samples while 69% tumors showed up-regulation of gene. Seventy five percent mammary tumors showed up-regulation of gene with highest fold change 8.69, 7.6, 5.63, 5.21 and 60% mast cell tumor showed up-regulation of gene with highest fold change 7.31. These results indicate that altered expression of tumor suppressor gene may be involved in malignant progression of tumors and might be helpful in the diagnosis and prognosis of canine tumors.</p>
K1 Dog, Mutation, Quantitative RT-PCR, CDKN1A, Carcinomas, Tumor suppressor gene
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-424