RT Journal T1 MEMORY ENHANCING EFFECT OF ANISE (PIMPINELLA ANISUM) WITH RESPECT TO ITS ANTIOXIDANT ACTIVITY IN ALBINO MICE A1 A. Mushtaq A1 R. Anwar A1 M. Ahmad JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 29 IS 2 SP 602 OP 610 YR 2019 FD 2019/04/01 DO DOI NA AB

The study was conducted to explore memory enhancing effect of Anise (Pimpinella anisum) to substantiate the scientific basis of its traditional use as a neurotic remedy. Aqueous (aqP.a) and n-hexane (nhP.a) fractions of crude Anise extracts were 1st investigated for phytochemical and antioxidant analysis and then administered to mice for behavioral (by using elevated plus maze (EPM), light dark test and hole board paradigms) and biochemical investigations. Flavonoids, tannins and phenols were found to be 127.43 ± 1.94, 201.04 ± 1.57 and 209.83 ± 1.30 mg/g, respectively for aqP.a and 125.33 ± 2.18, 74.66 ± 1.20 and 42.87 ± 1.65 mg/g, respectively for nhP.a. Values of IC50 were recorded as 37.48 and 166.53 µg/ml for aqP.a and nhP.a respectively. Moreover, aqP.a significantly (P < 0.001) improved inflexion ratios in EPM test and biochemical parameters like glutathiones, catalases and superoxide dismutases. The levels of acetylcholinesterase (AChE) and malondialdehyde (MDA) were significantly (P < 0.001) reduced by aqP.a as compared to nhP.a. The findings thus suggested that only aqueous extract of Anise improves memory both by preventing oxidative stress and by providing neuro protection to cholinergic pathways against AChE.

K1 Antioxidant, AChE, MDA, Dementia, Anise PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-232