RT Journal
T1 ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN NOD1 AND NLRP9 GENES WITH FECAL EGG COUNT TRAIT OF CHINESE AND BANGLADESHI GOAT BREEDS BEING NATURALLY INFECTED BY HAEMONCHUS CONTORTUS
A1 A. I. Omar
A1 L. Yin
A1 S. Inayat
A1 M. B. B. Alam
A1 W. Zhenyang
A1 T. Worku
A1 T. N. T. Thi
A1 M. O. Faruque
A1 K. Periasamy
A1 M. Samsuddin
A1 S. Zhao
A1 X. Du
A1 X. Liu
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 29
IS 2
SP 370
OP 378
YR 2019
FD 2019/04/01
DO DOI NA
AB <p>The study was conducted to identify relationships between single nucleotide polymorphisms (SNPs) in the&nbsp;<em>NOD1</em>&nbsp;and&nbsp;<em>NLRP9&nbsp;</em>genes and resistance to&nbsp;<em>H. contortus&nbsp;</em>infestation in goats in China and Bangladesh. A total of 507 unrelated goats from 6 different populations were sampled. Fecal egg count (FEC) was determined and genomic DNA was extracted from those goats.&nbsp;<a id="OLE_LINK98" name="OLE_LINK98"></a><a id="OLE_LINK97" name="OLE_LINK97">Touchdown PCR assays were used</a>&nbsp;to genotype each of the target SNP. An association study was carried out using a generalized linear model and R software after transforming the FEC as log10 (FEC + 25). All breeds of goat in Bangladesh had lower mean FEC than any of the Chinese breeds.<a id="OLE_LINK49" name="OLE_LINK49"></a><a id="OLE_LINK48" name="OLE_LINK48">&nbsp;Two</a><a id="OLE_LINK40" name="OLE_LINK40"></a><a id="OLE_LINK41" name="OLE_LINK41">&nbsp;single nucleotide polymorphisms (SNPs)</a>&nbsp;viz.&nbsp;<em>NOD1</em>_146_A&gt;G (p&lt;0.001) and&nbsp;<em>NLRP9</em>_43_A&gt;G (p&lt;0.003) were significantly associated with FEC trait after&nbsp;<em>H</em><em>.</em><em>&nbsp;contortus&nbsp;</em>infection.&nbsp;<a id="OLE_LINK80" name="OLE_LINK80"></a><a id="OLE_LINK81" name="OLE_LINK81"></a>Goats with the GG genotype for&nbsp;<em>NOD1</em>_146_A&gt;G (904.43 &plusmn; 140.65epg) and the AA genotype for&nbsp;<em>NLRP9</em>_43_A&gt;G (1129.57 &plusmn; 196.5 epg) had higher FEC than goats with the AA genotype (396.68 &plusmn; 67.22 epg) or the GG genotype (399.12 &plusmn; 106.4 epg) at the respective loci.&nbsp;<em>NOD1&nbsp;</em>and&nbsp;<em>NLRP9</em>&nbsp;genes were significantly associated with FEC and might be related to resistance to&nbsp;<em>H. contortus</em>&nbsp;infection in goats.</p>
K1 Goat, Haemonchus contortus, fecal egg count, parasitic resistance, SNP
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2019-JAPS-204