RT Journal
T1 CHARACTERIZATION OF PASTEURELLA MULTOCIDA IN DIFFERENT EGYPTIAN CHICKEN FLOCKS
A1 WafaaA.AbdEl-Ghany
A1 HananA.Ahmed andAliZ.Qandoos
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 28
IS 6
SP 1693
OP 1700
YR 2018
FD 2018/12/01
DO DOI NA
AB Inthepresentstudy,phenotypicandgenotypiccharacterizationof Pasteurella multocida(P. multocida)strainsfrom differentEgyptianlayersandbreederschickenflockswascarriedout.Organswerecollectedfrom55chickensflocks represented39layersand16broilersandlayersbreedersinEgyptduringtheperiod2016-2017.Sixbirdsweretakenfrom eachsuspectedflockwithatotalnumber330birdsthatrepresenting234and96freshlydeadlayersandbreederschickens, respectively.Suspectedorganscontaining P. multocidaweresubjectedforisolationandidentificationoftheorganism,as wellastestingofpathogenicityinmice.Moreover,genotypiccharacterizationof P. multocidaisolatewasdoneusing PolymeraseChainReaction(PCR).Capsularandsomatictypingwasdone.Thephenotypicandgenotypiccharacterization resultsrevealedthat6flockswerepositivefor P. multocidaoutof55ones(10.9%).Outof330deadchickens,36(10.9%) werepositivefor P. multocida.All P. multocidastrainswerepathogenicformice.Capsulartypingof P. multocidausing multiplexPCRshowedthatallstrainsweretypeA,whilesomatictypingrevealedpresenceoftypes1and3.Itcouldbe concludedthat P. multocidaiscirculatinginlayersandbreederchickenflocksinEgyptcreatingsevereeconomiclosses. multocida,PCRisused(Townsend et al.,1998,Sellyei et
K1 Pasteurella,PCR,LayersandBreeders,Egypt.
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-621