RT Journal T1 BIOINFORMATICS ANALYSIS OF CODON USAGE BIAS AND RNA SECONDARY STRUCTURES FOR SALT STRESS RESPONSIVE GENES IN ARABIDOPSIS THALIANA AND SOLANUM LYCOPERSICUM A1 B. Sadia A1 W. N. Hamzazai A1 I. A. Baloch A1 S. Kazmi A1 Asyia A1 P. S. Jan A1 S. Irfan JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 28 IS 5 SP 1532 OP 1536 YR 2018 FD 2018/10/01 DO DOI NA AB Salt stress is one of the most important abiotic stresses for agricultural crops and other plants. Salinity highly affects physical, physiological and metabolic responses of plants. Codon usage bias (CUB) and RNA secondary structures (RSS) were analyzed for salt stress responsive ( SSR) genes of Arabidopsis thaliana and Solanum lycopersicum. Four salt stress resistant genes SOS1 (salt overly sensitive 1), BZIP (basic lucien zipper protein), HVA22-like protein and CDPK1 (calcium-dependent protein kinase 1) of Arabidopsis thaliana as well as Solanum lycopersicum were compared for CUB and RSS. All genes expressed similarity for codon usage, as both plants showed to prefer more than 50% (65%, 50%, 60%, and 80%) similar codon usage for all four selected salt stress responsive genes. RSS analysis revealed that three SSR genes had significant difference in minimum free energy ( MFE) while two differed in number of loops and stems depicting overall mix behavior. These findings can further help to engineer the salt resistant genes by adjusting the codon usage through genetic screening and by using bioinformatics tools. of base pairing within a single molecule as well as in a set K1 Codon usage bias; Genetic code; Salinity responsive genes; Secondary structure. PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-537