RT Journal
T1 INVESTIGATIONS ON ONOSMA HISPIDUM WALL ROOT EXTRACTS FOR IN-VITRO ANTIDIABETIC, PROLIFERATIVE AND CYTOTOXIC EFFECTS
A1 M.Asghar
A1 M.Islam
A1 H.Saeed
A1 F.Imtiaz
A1 B.Saleem
A1 Z.Saleem
A1 S.Qamarand M.Iqtedar
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 28
IS 5
SP 1339
OP 1347
YR 2018
FD 2018/10/01
DO DOI NA
AB Theaimofthisstudywastoinvestigatetheimpactofvariousextractsofrootsof Onosma hispidumforanti-diabetes, proliferativeandcytotoxiceffectsusing in-vitroassays.Phytochemicalscreeningofpowderandextractswasdoneby employing standard methods. Anticancer activity was assessed by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazoliumbromide)andshorttermproliferationassayonHepG2cellline.Theantidiabeticpotentialwas determined byusing alpha-amylase inhibition assayand non-enzymatic glycosylation of haemoglobin assay. Data suggested that primary (CHO; 68.06 ± 0.19 and Proteins; 9.48 ± 0.27) and secondary metabolites (mg/g); max. Flavonoids (47.55 ± 0.13) in aqueous extract, max. Polyphenols (107.77 ± 0.57) in Pt. ether extract and max. glycosaponins(94.5±0.51)inchloroformextract,exhibitedvariationsamongvariousextractsobtainedfromrootsof Onosma hispidum.Petroleumetherandaqueousextractsexhibitedsignificantimpact(p<0.05)ontheproliferationof HepG2celllinesatdifferenttimepointscomparedtocontrol,whileincaseofMTTassay,nosignificantresults(p>0.05) wereobtainedafterovernightincubation.Improvedantidiabeticactivitywasexhibitedbypetroleumetherextract(70% inhibition)ascomparedtoAcarbosestandardusingalpha-amylaseinhibitionassay,withanIC of80µg/ml.Innon- 50 enzymatic glycosylation of haemoglobin assay, better potential was shown by chloroform extract with an IC of 50 35µg/ml,whencomparedtoastandard-Tocopherol.Thisstudyrevealedthattherootsof Onosma hispidumpossess nutritionalvalueandantidiabeticproperties,alongwithsignificantimpactoncell(HepG2)proliferation. and anti-mutagenic effects (Urquiaga and Leighton,
K1 Onosma hispidum, In-vitro,Polyphenols,Antidiabetic,Anticancer.
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-513