RT Journal T1 POLYMORPHISM OF β-LACTOGLOBULIN IN PRAMENKA SHEEP BREED IN BOSNIA AND HERZEGOVINA A1 A. Rustempašić A1 A.Dokso A1 E.Zečević A1 A. Hodžić A1 A.Hrković-Porobija A1 Z.Sarić A1 E. Miskoska–Milevska A1 Z. Popovski A1 M. Brka JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 28 IS 1 SP 337 OP 340 YR 2018 FD 2018/02/01 DO DOI NA AB

The aim of this study was to determinate allele frequency β-lactoglobulin in Pramenka breed sheep in Bosnia and Herzegovina. The DNA was extracted from blood of 283 sheep, collected from four flocks around Sarajevo, Bosnia and Herzegovina. The gene β-lactoglobulin was amplified using polymerase chain reaction – restriction fragment length polymorphism (PCR–RFLP) technique. Two genetic variants (A and B) and three genotypes (AA, AB and BB) of β-lactoglobulin have been identified. Genotype frequencies of β-lactoglobulin were with the following values: AA (0.325), AB (0.445), and BB (0.229), while the frequencies of allele A and B were 0.547 and 0.453 respectively. The highest frequency of genotype AA is present in the location Treskavica (38%), genotype AB in the location Vlašić (52%), while the genotype BB is mostly present in location Bjelašnica (29%). The population of Pramenka breed sheep was in Hardy-Weinberg equilibrium. It is expected that in a future the programs in sheep breeding in Bosnia and Herzegovina certainly would take into account the genetic variants of β-lactoglobulin that could contribute to better production and economic benefit.

K1 β-lactoglobulin;genetic polymorphism; Pramenka breed PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-41