RT Journal T1 BIOLOGICAL EVALUATION OF LOCALLY PRODUCED RECOMBINANT PHYTASE IN BROILER CHICKS A1 F.Sabir A1 M.Tayyab A1 A.R.Awan A1 B.Muneer A1 A.S.Hashmi A1 M.Wasim andS.Firyal JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 28 IS 3 SP 946 OP 950 YR 2018 FD 2018/06/01 DO DOI NA AB Currentstudydealswiththeproductionoflocallycharacterizedrecombinantthermostablephytasefrom Thermotoga naphthophila(PHY )anditsbiologicalevaluationinpoultrybroilerchicks.ThePHY wasproducedfrom16-literLB TN TN mediumusingBL21CodonPluscellshavingpET-21acontainingphytasegenefrom Thermotoga naphthophila.Atotal of135birdsweredividedinto5groupseachhaving27birds.GroupAservedasnegativecontrol,groupB,CandD weregivenfeedsupplementedwith500,1000and1500IU/kgoflocallyproducedrecombinantphytasewhilegroupE servedaspositivecontrol.Theresultsdepictedthatlocallyproducedrecombinantphytaseshowedsignificanteffecton birdsweightgain,feedintakeandfeedefficiencyratio.Presenceof1000IU/kgofphytaseresultedintheweightgainin rd th th 3 ,4 and5 weekoftrialfrom504.77±1to533.53±1.4,767.9±2to823.73±2.84and999.8±7.66to1122.8±2g respectivelywhencomparedwiththecontrol.ThegroupB,CandDshowedanaveragefeedintakeof2710,2780and 2790gascomparedto2669gforgroupA.Thesupplementationof1000IU/kgoflocallyproducedphytaseinfeed resultedintheimprovementoffeedconversionratiofrom2.6to2.4.ThestudydemonstratedthatPHY issuitablefor TN poultryfeedindustryanditsdomesticproductionwillcontributeintheeconomicavailabilityoflocallyproducedphytase forthepoultryfeedindustry. TN supplementationinmonogastricdiets.Thefeedpelleting K1 PHY ,PoultryTrial,RecombinantThermostablePhytase, Thermotoga naphthophila,Phytate PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-332