RT Journal T1 IN PROCESS QUALITY CONTROL FACTORS AFFECTING THE QUALITY OF LOCALLY PREPARED SALMONELLA GALLINARUM ANTIGEN A1 Z. Malik A1 A. Ahmad A1 A. A. Anjum A1 A. Aslam A1 M. Z. Shabbir A1 A. Ghafoor A1 J. Muhammad A1 M. Avais A1 N. Ahmad A1 M. H. Saleem A1 M. Rabbani JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 28 IS 2 SP 656 OP 659 YR 2018 FD 2018/04/01 DO DOI NA AB

Seroserveillance of fowl typhoid using imported antigen is not cost effective, more over import of meager quantity of antigen is always not possible. Therefore, Fowl typhoid antigen was locally prepared and evaluated for sensitivity and specificity. Effect of preservatives and temperature on sensitivity of local antigen was also studied. Of three test antigens (6, 8 & 10 million cells/ml), one with 10 million cells showed visible agglutination with known antisera upto log 10-3 dilution (3000 cells/0.3 ml) as compared to other two antigens (8, 6 million cells/ml) that showed agglutination upto 10-2 dilution (24000cells/0.3ml, 18000cells/0.3ml respectively). Antigens stored at -20°C and 25°C showed visible agglutination up to 90 and 30 days post preservation respectively in presence of both preservatives thimerosal sodium and sodium azide, separately, while no change in agglutination reaction was observed for antigen stored at 4°C throughout the whole storage period of 6 months. No cross reactivity of local antigen with antisera against Mycoplasma gallisepticum, Mycoplasma synoviae and negative control serum was observed. On comparison, local antigen proved equally efficient as imported antigen with known positive and negative control sera and may be used for screening of Salmonella gallinarum infection in poultry.

K1 Salmonella gallinarum, Fowl typhoid, Diagnosis, Antigen, Pakistan PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2018-JAPS-239