RT Journal
T1 RECOMBINANT LENTIVIRUS VECTOR TARGETING BOVINE LXRΑ MRNA AND ITS SILENCING EFFECTS ON BOVINE MUSCLE SATELLITE CELLS
A1 Y. Liu
A1 P. Lib
A1 L. Zhao
A1 Z. Zhao
A1 Y. Li
A1 M. Liu
A1 X. Yang
A1 L. Zan
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 27
IS 6
SP 1847
OP 1851
YR 2017
FD 2017/12/01
DO DOI NA
AB <p>The liver X receptor &alpha; (<em>LXR&alpha;)&nbsp;</em>is a member of the nuclear hormone receptor superfamily which could regulate the transcription of the genes involved in cholesterol transportation. In this study, Lentiviral vector containing<em>&nbsp;LXR&alpha;&nbsp;</em>shRNAs were constructed and transferred in bovine muscle satellite cells. The results indicated that&nbsp;<em>LXR&alpha;&nbsp;</em>down regulated&nbsp;<em>PPAR&alpha;</em>,&nbsp;<em>PPAR&gamma;</em>,&nbsp;<em>ABCA1</em>,&nbsp;<em>LPL</em>,&nbsp;<em>ApoE&nbsp;</em>genes after 48 h post-pLenti-03 viruses' infection. The silencing effects of bovine&nbsp;<em>LXR&alpha;&nbsp;</em>gene on related were significant. This work identifies bovine<em>&nbsp;LXR&alpha;&nbsp;</em>role in cholesterol metabolism.</p>
K1 bovine LXRα gene, lentivirus, vector construction, gene silencing
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-234