RT Journal T1 EFFECTS OF DIFFERENT CONCENTRATIONS OF BAP AND NAA ON MICROPROPAGATION OF Crambe orientalis L. var. orientalis L. A1 Y. Ceylan A1 F.A. Özdemir A1 A. S. Bülbül JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 27 IS 5 SP 1671 OP 1677 YR 2017 FD 2017/10/01 DO DOI NA AB

Crambe L. is a species of the Brassicaceaefamily. It is an important industrial oil plant with high erusic acid content. The objective of this study was to develop an efficient micropropagation protocol for Crambe orientalis L. var. orientalis L. species using different concentrations of BAP (6 - benzylaminopurine)–NAA (α - naphthalene acetic acid) hormone combinations. Seeds of Crambe orientalis var. orientalis were germinated in vitro. Cotyledon node and hypocotyl parts from fourteen-days seedlings were used as explant sources. The explants were cultured on MS media containing 0.25, 0.50, 1.00 and 2.00 mg/L BAP and 0, 0.25 and 0.50 mg/L NAA. Effects of hormone concentrations on mean callus regeneration percentage (%), mean shoot regeneration percentage (%), mean number of shoots per explant and mean length of shoots were determined. Callus formation and shoot regeneration resulted in all hormone concentrations used for the hypocotyl and cotyledon explants in our study. All explants were rooted on MS media containing 1.00 and 2.00 (mg/L) NAA.

K1 BAP, Brassicaceae, callus, Crambe orientalis L. var. orientalis L., micropropagation, NAA PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-211