RT Journal
T1 ASSESSMENT OF THREE DIFFERENT ENDOMETRIAL CYTOLOGICAL SAMPLING METHODS IN POSTPARTUM BEEF COWS
A1 N. Salah
A1 N. Yimer
A1 H. Wahid
A1 Y. Rosnina
A1 A. Khumran
A1 E. Mahdi
A1 F. Baiee
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 27
IS 5
SP 1515
OP 1521
YR 2017
FD 2017/10/01
DO DOI NA
AB <p>The aim of this study was to assess three different cytological endometrial sampling methods used to estimate&nbsp;<a title="Polymorphonuclear leukocytes" href="https://en.wikipedia.org/wiki/Polymorphonuclear_leukocytes">polymorphonuclear leukocytes</a>&nbsp;(PMN) under high power field (HPF) microscopy and to determine subclinical endometritis in postpartum beef cows. Forty beef cows aged&nbsp; 3-7 years were sampled at week three and four after calving by endometrial cytology methods. The cytological sampling methods used included;&nbsp; cotton swab (CS), cytobrush (CB) technique, and low volume flush (LVF), respectively. The mean&nbsp; PMN counts at&nbsp; the third week was higher (p&lt;0.01) (12.2 cells HPF-1<span dir="rtl">&nbsp;</span>than on the fourth week (4 cells HPF-1). The average PMN counts using CB alone was significantly&nbsp; higher (11.3 cells HPF-1)&nbsp; than CS (7 cells HPF-1) and LVF ( 6 cells HPF-1) methods. Smears from CB had more endometrial cells (58.55 cells HPF-1) at HPF, which was significantly higher (p<span dir="rtl">&nbsp;</span><span dir="rtl">&gt;</span><span dir="ltr">&nbsp;</span>0.01)&nbsp; than CS and LVF methods. Both CB and CS methods yielded more intact cells (62.4 % and 61.9 %) (p&nbsp;<span dir="rtl">&gt;</span><span dir="ltr">&nbsp;</span>0.01) than LVF (52.4 %). The prevalence of subclinical endometritis in the beef cows between 22 and 28 days postpartum using a threshold value of&nbsp;<span dir="rtl">&le;</span><span dir="ltr">&nbsp;</span>8 % by cytobrush method was 12.5%, which is considered low. In conclusion, CB method was found to be better and effective technique in comparison to other cytological methods used in obtaining endometrial cytology samples.</p>
K1 endometritis, postpartum,  cytology, neutrophils
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-191