RT Journal T1 DROUGHT-INDUCED TRANSCRIPTION OF RESISTANT AND SENSITIVE COMMON MILLET VARIETIES A1 R. Y. Wang A1 H. G. Wang A1 X. Y. Liu A1 S. Lian A1 L. Chen A1 Z. J. Qiao A1 C. E. McInerney A1 L. Wang JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 27 IS 4 SP 1303 OP 1314 YR 2017 FD 2017/08/01 DO DOI NA AB

As a well-adapted plant to dry soil and short growing seasons, common millet holds great potential as a drought-resistant crop. Here, we performed a comparative RNA-Seq analysis of two common millet genotypes, Huangmizi (tolerant) and Zhenyuandamizi (sensitive) grown under stress conditions (20% Polyethylene Glycol 6000 with an average molecular weight of ～5,400-7,000 solution). Leaves of seedling plants were harvested from a control and also after 1 and 3 h stress treatment and pooled for RNA isolation. Using Illumina paired-end sequencing, approximately 46.5 million clean reads were generated. Following a de novo assembly, a total of 42,240 Unigenes were obtained. A total of 2,301 SSRs and 1,447,148 SNPs were also identified and 75% of Unigenes were annotated. A total of 21,556 and 8,304 Unigenes aligned to the GO and COG databases, respectively. The GO classification showed that the Unigenes were distributed into three categories with 74.30% having biological functions, 76.51% having molecular functions and 79.30% having cellular-level functions. By cross-referencing against KEGGs, 5,535 Unigenes were assigned to 63 metabolic pathways. Additionally, 701 DEGs including 187 up-regulated and 514 down-regulated genes were detected. Transcripts identified in this study will accelerate our understanding of the molecular mechanisms of drought tolerance in common millet.

K1 Common millet (Panicum miliaceum L.), Drought, Transcription PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-165