RT Journal T1 MOLECULAR CHARACTERIZATION OF A BEGOMOVIRUS AND ASSOCIATED SATELLITES FROM COTTON (GOSSYPIUMHIRSUTUM FROM DERA GHAZI KHAN DISTRICT OF PAKISTAN A1 A. Akram A1 K. Hussain1 A1 N. Nahid A1 Mahmood-ur-Rahman A1 A. Nasim A1 S. Shaheen JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 27 IS 4 SP 1245 OP 1255 YR 2017 FD 2017/08/01 DO DOI NA AB

Cotton leaf curl disease is a major threat to cotton production in tropical and subtropical regions of the world. Begomoviruses are whiteflies-transmitted single-stranded DNA viruses belonging to the family Geminiviridae thatcause leaf curl disease in cotton. In present study, a strain of tomato leaf curl betasatellite and Xanthium strumarium alphasatellite have been found associated with Cotton leaf curl Kokhran virus in Gossypium hirsutum. A begomovirus and associated molecules were amplified, cloned and sequenced. Partial sequence analysis for begomovirus showed 98.2% sequence identity with an isolate of Cotton leaf curl Kokhran virus (HQ257374). The partial sequence of betasatellite showed highest sequence identity of 96.4% with tomato leaf curl betasatellites (EF068245, FR819710). The complete sequence for alphasatellite showed 98.2% sequence identity with Xanthium strumarium alphasatellite (HF547408). This is the first report that tomato leaf curl betasatellite have been found in cotton and after second epidemic of cotton leaf curl disease this is the first report of parent strain of Cotton leaf curl Kokhran virus in the field.

K1 Cotton, Begomovirus, Betasatellite, Alphasatellite, Cotton leaf curl disease PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-158