RT Journal
T1 EXPRESSION OF SMALL HEAT SHOCK PROTEIN23 ENHANCED HEAT STRESS TOLERANCE IN TRANSGENIC ALFALFA PLANTS
A1 Ki-Won Lee
A1 Md. Atikur Rahman
A1 Gi Jun Choi
A1 Ki-Yong Kim
A1 Hee Chung Ji
A1 Tae Young Hwang
A1 Sang-Hoon Lee
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 27
IS 4
SP 1238
OP 1244
YR 2017
FD 2017/08/01
DO DOI NA
AB <p>Heat shock proteins (Hsps) are key components contribute to cellular homeostasis under stressful conditions. They function as molecular chaperone involved in protein folding, plant development, signal transduction and protecting plants against several stresses. In this study, we developed transgenic alfalfa by&nbsp;<em>Agrobacterium</em>-mediated genetic transformation, and integration and expression of&nbsp;<em>MsHsp23</em>was confirmed by polymerase chain reaction (PCR), Southern and Northern blot analyses. Transgenic (Tg1, Tg3, Tg4 andTg7) and non-transgenic (NT) alfalfa plants were exposed to heat (42 &deg;C)treatment for 24 h. Compared to NT control plants, Tg plants showed less wiltingthat maintained leaves color compared to NT plants. The expression of&nbsp;<em>MsHsp23</em>gene and the ascorbate peroxidase (APX) levels were higher in&nbsp;<em>MsHsp23</em>alfalfa lines relative to NT plants. The result indicates the chaperone activity of&nbsp;<em>MsHsp23</em>genethat allows for the induction of APX in transgenic alfalfa. We conclude that high level of&nbsp;<em>MsHsp23</em>withAPX activity enhanced tolerance to heat stress in transgenic alfalfa plants.</p>
K1 MsHsp23, Transgenic, Alfalfa, Heat stress, Tolerance
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2017-JAPS-157