RT Journal T1 CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF Eruca sativa SEEDS AGAINST PATHOGENIC BACTERIA AND FUNGI A1 H. Rizwana A1 Mona. S. Alwhibi A1 F. Khan A1 D. A. Soliman JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 26 IS 6 SP 1859 OP 1871 YR 2016 FD 2016/12/01 DO DOI NA AB

The objective of the present study was to investigate the antimicrobial activity of chloroform, ethyl acetate, acetone, methanol and ethanol extracts of Eruca sativa seeds growing in Saudi Arabia. Major chemical constituents and functional groups of E. sativa extract were determined by Gas Chromatography -Mass Spectrometry and Fourier Transform-Infra Red Spectroscopy analysis. Seed extracts showed potent antimicrobial activity against pathogenic bacteria and fungi. Ethyl acetate extract was highly efficient in controlling the growth of Staphylococcus aureus, Bacillus subtilus, Methicillin resistant Staphylococcus aureus, Streptococcus pyogenes and Escherichia coli. Maximum zone of inhibition was exhibited by S. aureus (26mm).Similarly, methanol extract showed strong inhibitory effects on mycelial growth of all the fungi tested except for Colletotrichum gleosporoides. Maximum mycelial inhibition (82%) was recorded for Colletotrichum musae. The minimum inhibitory concentration and minimum bactericidal concentration results demonstrated the efficiency of the extracts screened. Furthermore, micrographs from scanning electron microcopy revealed the damaging effects of extracts on cell morphology as blebs, indentation and complete lysis at 0.8mg/ml. GC-MS analysis of ethyl acetate and methanol extracts has led to identification of important compounds  which are known antifungals and antibacterials .

K1 Antibacterial, Antifungal, GC-MS analysis, FT-IR, SEM, Cell lysis PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-243