RT Journal T1 DETERMINATION OF IMIDACLOPRID RESIDUES IN RICE FROM VARIOUS DISTRICTS OF PUNJAB USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY A1 A. Niaz A1 R. A. Sial A1 M. Yaseen A1 G. A. Mand A1 M. H. Javed A1 E. Ahmad A1 R. Ahmad A1 M. Rahim JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 26 IS 1 SP 170 OP 176 YR 2016 FD 2016/02/01 DO DOI NA AB

Rice is Pakistan’s major export crop and injudicious use of several pesticides by rice growers is making it all difficult for exporters to get their shipments clear regarding pesticide residues. In continuation of systematic research for determination of pesticide residues, rice samples were collected from key growers of important rice growing areas of Punjab including Gujranwala, Sialkot, Hafizabad, Narowal, Sheikhupura, Mandi Bahaudin, Pasrur and Jhang. Imidacloprid is a systemic insecticide with seed, foliar and soil uses to control sucking pests. Newly proposed maximum residual limit (MRL) for imidacloprid in rice by European food safety authority is 1.5 µg g-1. High performance liquid chromatography with photo diode array detector was employed for imidacloprid residues analysis. Method validation data showed a recovery percentage of 86±6.12%, 115±3.53%, 108±6.01%, 93±3.53% and 93±3.84% for fortification levels of 0.01, 0.05, 0.1, 0.5 and 1 µg g-1 rice sample, respectively. Residues analysis data showed that most of the randomly selected samples carried imidacloprid residues. Three samples from district Sialkot (1.63, 2.22 and 2.74 µg g-1) while one sample each from Gujranwala (2.63 µg g-1) and Narowal (1.88 µg g-1) exceeded the MRL. Imidacloprid residues were recorded in the range of 0.47 to 2.63 µg g-1, 0.95 to 2.74 µg g-1, < 0.01 to 1.16 µg g-1, 0.43 to 1.88 µg g-1, 0.01 to 0.82 µg g-1, 0.07 to 0.76 µg g-1, <0.01 to 0.14 µg g-1 and <0.01 to 0.1 µg g-1 rice in samples from Gujranwala, Sialkot, Hafizabad, Narowal, Sheikhupura, Mandi Bahaudin, Pasrur and Jhang, respectively. The information would be beneficial for rice exporters. Moreover, extension workers and their field staff should translate to the rice growers the threat their produce would be facing in modern international markets.

K1 Imidacloprid Residues, Rice, Punjab, Pakistan, HPLC PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-22