RT Journal T1 IN VITRO IMPROVEMENT OF CHARACTERS IN ROSA SPP. THROUGH COLCHICINE A1 M. M. Q. Baig A1 T. Ahmad1 A1 I. A. Hafiz A1 N. A. Abbasi A1 M. J. Tareen JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 26 IS 6 SP 1666 OP 1674 YR 2016 FD 2016/12/01 DO DOI NA AB
Flower size and color variation are the main attributes in the floriculture industry that affect the consumers demand. Rosa gruss an teplitz and Rosa centifolia are the fragrant rose species known for their wonderful aesthetic beauty. However, they are limited in use due to their small sized flowers and no color variegation. In order to improve characters in rose species, shoot tips taken from micropropagated plants were incubated for three different periods of 5, 7 and 11 days in MS medium containing different levels (0,100, 150, 300 and 450 mg l-1) of colchicine. Rosa gruss an teplitz and Rosa centifolia yielded maximum plant height (15.12 and 17.23cm), number of nodes (4.80 and 6.93), internodal distance (3.15 and 2.48 cm), leaf area (5.26 and 10.50 cm2) and flower size (6.24 and 5.78cm) respectively at colchicine level of 450 mg l-1 with incubation period of 11days. Colchicine also induced variegation and variation in flowers of Rosa gruss an teplitz. Genetic variation was analyzed and dendrogram was developed to assess the relationship with parent material. The findings of this work add to the ongoing efforts to improve the characters through induction of mutation in rose flowers.
K1 Rosa gruss an teplitz, Rosa centifolia, colchicine, RAPD, variation PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-219