RT Journal T1 IS QUERCETIN ACCUMULATING IN EGGS USING FLAVONOIDS-ENRICHED POULTARY FEED? A1 S. Fayyaz A1 S. Saddique A1 H. Khalid A1 S. Naureen A1 M. Salman A1 M. Islam A1 F. Zahid A1 S. Naheed A1 M.T. Khan A1 S. Amjad A1 S. Qamar A1 S. Ahmad JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 26 IS 5 SP 1479 OP 1485 YR 2016 FD 2016/10/01 DO DOI NA AB

Flavonoids-enriched feed, besides enhancing poultry performance like increase in meat’s shelf life, egg laying rate, egg shell strength, protein contents and Haugh unit and decrease in fats contents of yolk, may result in accumulation of active components such as quercetin in eggs, which has not yet verified. Therefore, the present study describes a simple ultraviolet spectrophotometric method for the determination of quercetin in egg white and yolk. The analyte was extracted from both parts of the egg and specifically detected at 370 nm. The detection was not compromised by degradation products of the analyte produced under stress conditions. Beer’s range was found to be 2.50-37.50 µg/mL with correlation coefficient approaching 0.998. LOD and LOQ were found to be 0.15 and 0.47 µg/mL, respectively. The recovery, intraday accuracy and inter-day accuracy were found to be (98.49-100.89%), (99.12-101.78) and (98.13-104.53%), respectively. The method showed precision because relative standard deviation was less than 5%. The fortified and domestic eggs showed the presence of quercetin in both the parts. The results of the present study indicate that quercetin accumulates in egg, and the method is simple, accurate, repeatable and reproducible.

K1 Quercetin; Poultry feed; Flavonoids; Ultraviolet spectroscopy; Eggs PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-194