RT Journal
T1 HEPATOPROTECTIVE ACTIVITY OF OPUNTIA DILLENII (KER GAWL.) HAW. FRUIT PULP EXTRACT AGAINST CADMIUM INDUCED TOXICITY IN MICE
A1 A. Shah
A1 K. R. Ahmad
A1 N. Aziz
A1 S. Rahim
A1 S. Batool
A1 S. Suleman
A1 T. Abbas
A1 M. A. Kanwal
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 26
IS 5
SP 1471
OP 1478
YR 2016
FD 2016/10/01
DO DOI NA
AB <p>The present study was conducted to evaluate the hepatoprotective activity of&nbsp;<em>Opuntia dillenii&nbsp;</em>(Ker Gawl.) Haw. (OD) fruit extract in mice (<em>Mus musculus</em>) against Cadmium (Cd) induced hepatic-histopathology. Thirty albino mice were randomly distributed in three groups (n=10). Control(C) (untreated group) maintained on regular supply of food and drinking water. The Cd and Cd+OD groups were given 50ppm Cd ions in drinking water for 15 days and Cd free water for the next 7 days. The Cd+OD group additionally received 0.2ml of OD fruit extract (once daily) by gavage on days 16-22. The histological slides in Cd group revealed scattered aggregations of hepatocytic debris in the wavy hepatic cords with little sinusoidal spaces in between. Mostly the hepatocytes were swollen containing enlarged nuclei and vacuolated cytoplasts. In Cd+OD group these pathological signs were partly reversed additionally scattered aggregations of hepatoblastic progenitor cells were also visible. Mean cross-sectional areas of central lobular vein, hepatocyte and hepatocytic nucleus were significantly (p&le;0.05) higher in Cd (6368.34&plusmn;5.87&micro;2, 400.9&plusmn;20.01&micro;2 and 82.64&plusmn;9.07&micro;2 respectively) than the control (3280.20&plusmn;1.83&micro;2, 184.10&plusmn;11.77&micro;2 and 65.86&plusmn;7.1&micro;2) and Cd+OD (3824.64&plusmn;2.17&micro;2, 389.51&plusmn;26.74&micro;2 and 76.14&plusmn;6.06&micro;2) groups whereas, the mean number of &nbsp;hepatocytes per hepatic cord were significantly (p&le;0.05) higher in control (11.60&plusmn;.37) than Cd+OD (10.84&plusmn;.43) and Cd (8.56&plusmn;.32) groups; however the mean number of progenitor cells perunit area were significantly higher in Cd+OD (6.76&plusmn;.26) than control (4.43&plusmn;.23) and Cd (2.32&plusmn;.21)group. These findings reflect the curative role of the&nbsp;<em>O. dillenii</em> fruit extract on the hepato- histopathological changes of Cd exposure in mice, clearly indicating its hepato-protective medicinal importance for similar possible human benefits.</p>
K1 Opuntia dillenii, Cadmium toxicity, Hepatohistopathology, Mianwali
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-193