RT Journal T1 SEQUENCING OF THE NFE2L1 GENE IN SHEEP AND EVALUATION INFLUENCE OF GENE POLYMORPHISMS ON MEAT PRODUCTION A1 V. Trukhachev A1 V. Skripkin A1 A. Kvochko A1 A. Kulichenko A1 D. Kovalev A1 M. Selionova A1 M. Aybazov A1 O. Yatsyk A1 A. Krivoruchko JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 26 IS 5 SP 1262 OP 1267 YR 2016 FD 2016/10/01 DO DOI NA AB

The aim of this study was to investigate influence of the NFE2L1 gene polymorphisms on some parameters of meat production in sheep. Polymorphisms of the NFE2L1 gene were detected using NimbleGen sequencing technology (Roche, USA). The effect of polymorphisms identified was investigated in Manych Merino sheep breed. In coding and regulatory parts of gene 11 single nucleotide polymorphisms (SNP) were detected. These are the substitutions: c.-1092T>C, c.-703C>T, c.582C>T, c.788C>T, c.864C>T, c.*177C>G, c.*179C>T, c.*181C>A, c.*182G>T, c.*187G>T and c.*188G>T. Five of these SNP has been identified for the first time. Three of founded SNP c.788, c.-1092 and c.*188 have positive effect on vital meat production indicators in sheep. Another SNP c.864 have a negligible effect on investigated parameters. Consequently, the determination of allelic variants of the NFE2L1 gene may be used in marker-assisted selection. In future will evaluate the influence of founded SNP on slaughter parameters of meat production.

K1 NFE2L1, Sheep, Manych Merino, SNP, Sequence PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2016-JAPS-165