RT Journal T1 CALLUS INDUCTION, IN VITRO SHOOT REGENERATION AND HAIRY ROOT FORMATION BY THE ASSESSMENT OF VARIOUS PLANT GROWTH REGULATORS IN TOMATO (Solanum lycopersicum Mill.) A1 S. H. Shah A1 S. Ali A1 S. A. Jan A1 J. Din A1 G. M. Ali JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 2 SP 528 OP 538 YR 2015 FD 2015/04/01 DO DOI NA AB

In vitro morphogenesis is greatly influenced by plant growth regulators (PGRs). The effect of different plant growth regulators was assessed on callus induction, in vitro shoot regeneration and hairy root formation in tomato. The maximum callus induction frequency was recorded culturing hypocotyls, while in vitro shoot regeneration frequency was significantly higher when leaf discs were used as explants. The highest callus induction frequency (67.48%) was recorded in cv. Rio Grande followed by Roma (62%) and Moneymaker (58.23%) on MS basal media along with 2.0 mg/l IAA and 2.5 mg/l BAP. Similarly, the highest in vitro shoot regeneration frequency (69.6 and 65.3%) was recorded in Rio Grande and Roma on MS media fortified with 0.1 mg/l IAA, 1.0 mg/l ZEA and 2.0 mg/l BAP, while in case of Moneymaker, the highest in vitro shoot regeneration frequency (67.3%) was obtained on MS media enriched with 0.1 mg/l IAA and 3.0 mg/l BAP. The highest hairy root formation frequency (100, 91.72 and 93.74%) was achieved in cvs. Rio Grande, Moneymaker and Roma, respectively on MS media supplemented with 0.1 mg/l IAA, 2.0 mg/l ZEA and 2.0 mg/l BAP. These optimized procedures would be applicable to other genotypes of tomato for the development of cultivars tolerant to abiotic stresses developed via Agrobacterium mediated transformation system.

K1 Hypocotyls; In vitro morphogenesis; Leaf discs; Plant growth regulators, Hairy roots PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-72